![]() ![]() melanogaster first instar larval ventral nerve cord at four different zoom levels (A–D where B shows the close up of the blue rectangled area in A etc.). The movie shows in each frame a single section from the rigidly aligned TEM section series, imaged as mosaic of overlapping tiles, of the D. Walk through reconstructed large ssTEM mosaic of third instar larval ventral nerve cord. In order to emphasize the visualization effect all tiles within section are initially placed at the same location discarding their known configuration within section. The global optimization proceeds section by section and at each step distributes the registration error equally across the increasing set of tiles. The corresponding SIFT features that connect images within section and across section are shown as green dots, the residual error of their displacement at a given iteration of the global optimizer is shown as cyan line (iteration number and minimal, average and maximal error are shown in lower left corner). The video visualizes the process of reconstruction of such large section series on seven exemplary sections. The ventral nerve cord of Drosophila first instar larva was sectioned and imaged in electron microscope as a series of overlapping image tiles. Visualization of SIFT mediated stitching of large ssTEM mosaics. Between 23 rd of March to the 3 rd of April there was a Fiji hackathon in Dresden marked by increased developer activity that carries over the period after the hackathon ended – the so called “hackathon effect”. The class hierarchy is visualized as a dynamic tree, the developers are flying pawns that extend rays to classes which they newly created or into which they introduced changes. The video produced using gource tool in Git visualizes the changes to Fiji source code repository from March 15 th 2009 until May 16 th 2009. The future of Fiji as application layer of ImageJ2 An estimate of Fiji users based on wiki traffic NIHMSID 517436 Supplement: Supplementary Figure 1. ![]()
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